CasNo： 9011-18-1
Molecular Formula： (C6H7Na3O14S3)n
Product Description
Dextran sulfate sodium salt (DSS) is a polysaccharide derivative containing sulfate, which is used for many purposes according to the molecular weight of biomedical and clinical research (refer to the table below). An important feature of DSS is to induce colitis in mice and rats when drinking water. DSS combined with medi chain length fatty acid in large intestine to induce enteritis. The advanced dextran sulfate soil salt (Mw = 36000-50000) of MP biomedicals is the most used product in famous academic journals such as papers. So far, more than 10000 papers have cited the DSS of MP bio.
Dextran sulfate sodium (DSS) is one of the most common and effective compounds to induce ulcerative colitis in animals. DSS colitis model, like colorectal cancer in patients with long-term ulcerative colitis, is widely used in the study of colorectal cancer caused by colitis.
MP biomedicals provides dextran sodium sulfate (3600-50000) of gold standard equivalent to colitis inducing reagent and azoxymans (AOM) for inducing colorectal cancer.
The DSS of MP biomedicals has high efficacy and is one of the most used DSS products in the scientific literature. So far, more than 10000 scientific literatures have cited the DSS products of MP biomedicals and successfully established the animal model of colitis / colorectal cancer.
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
The animal models of acute / chronic ulcerative colitis and chronic colorectal cancer were established.
Specifications
| Product Name | Dextran sulfate sodium salt |
| Alternate Names | DSS, Dextran Sodium Sulfate |
| Application Notes |
Dextran sulfate sodium (DSS) is one of the most common and effective compounds to induce ulcerative colitis in animals. DSS colitis model, like colorectal cancer in patients with long-term ulcerative colitis, is widely used in the study of colorectal cancer caused by colitis. MP biomedicals provides dextran sodium sulfate (3600-50000) of gold standard equivalent to colitis inducing reagent and azoxymans (AOM) for inducing colorectal cancer. The DSS of MP biomedicals has high efficacy and is one of the most used DSS products in the scientific literature. So far, more than 10000 scientific literatures have cited the DSS products of MP biomedicals and successfully established the animal model of colitis / colorectal cancer. |
| Boiling Point | 310 ° C |
| CAS # | 9011-18-1 |
| Grade | Colitis Grade |
| Melting Point | 92 ° C |
| Molecular Weight | 36,000 - 50,000 Da |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| PH | (1% aqueous solution) 5-7.11 |
| References |
Kinchen, J. Structural Remodeling of the Human Colonic Mesenchyme in Inflammatory Bowel Disease. Cell. 2018, 175, 372-386. Mahalhal , A. Oral iron exacerbates colitis and influences the intestinal microbiome, PLOS ONE. 2018, 13, e0202460. Dokoshi, T. Hyaluronidase inhibits reactive adipogenesis and inflammation of colon and skin. JCI Insight. 2018, 3, e12307. Zhu, H. RNA virus receptor Rig-I monitors gut microbiota and inhibits colitis-associated colorectal cancer. J. Exp. Clin. Cancer Res. 2017, 36, 2. Monticelli, L. IL-33 promotes an innate immune pathway of intestinal tissue protection dependent on amphiregulin–EGFR interactions. PNAS. 2015, 112, 10762-10767. |
| Solubility | 100 mg/mL (decreases with increasing of MW) |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| Vapor Pressure | 2.6 x 10-4 mm Hg at 25 ° C (Estimated) |
Superiority
The traditional production method of Dextran involves bacterial fermentation and alcohol precipitation, which is low in efficiency and costly. Herbon International’s proprietary enzymatice synthesis technology can synthesize the required molecular weight Dextran products. This technology effectively avoids the bacterial pollution caused by the traditional bacterial fermentation method, and simplify the production process. No organic solvents are used during the process. We use a nano filtration purification system to remove impurities, which guarantees high-purity products, ensuring our product quality achieves international standards, also provide high-quality substrate raw materials for our derivative products.
Based on our competitive advantage in Dextran products, we can accommodate customisationstailormake Dextran Sulfate Sodium Salt products according to the needs of client’s needs, including molecular weight and other quality requirements.
Dextran Sulfate Sodium Salt Product List
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DS-T1 |
1,000 |
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DS-T3 |
3,000 |
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DS-T5 |
5,000 |
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DS-T8 |
8,000 |
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DS-T10 |
10,000 |
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DS-T20 |
20,000 |
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DS-T40 |
40,000 |
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DS-T500 |
500,000 |
Frequently Asked Questions
A: Yes, generally 2 days cause epithelial barrier destruction, you can observe it on Day 3, Day 5, Day7; A pathological examination is suggested to perform.
A: The daily water intake value is: for mice is 7~10ml/day, and for rats is 11ml/100g body weight/day.
Q: Literature reports on the length of modeling time for AOM/DSS models are not consistent. Are there other models of bowel cancer, like induced by DSS alone?
A: There are other models, but each model serves a different purpose. DSS can induce intestinal cancer itself, but the induction time is long. References: Nature Protocols; 2007, 2 (8): 1998-2004.
A: DSS is a dextran polymer with average molecular weight. There is a difference in molecular weight between batches, and molecular weight has an influence on enteritis modeling. In general, DSS batches are relatively stable, but there are some large batch differences in some batches. Therefore, it is suggested that customers purchase enough quantity products of the same batch according to their own experimental requirements or conduct pre-experiments before changing batches.
A:It is normal for some animals to have a slow reaction time and even lose weight on the fifth day after drinking DSS. If no symptoms appear for 7 days, the experiment is repeated and the drinking concentration of DSS is suggested to increase by 0.5~1%.
A: There are many factors affecting DSS modeling, among which the breeding environment of animals also has a great influence.
A: Excessive drinking concentration of DSS will lead to intestinal bleeding. Mild intestinal bleeding has no significant effect. Excessive bleeding is suggested to reduce the concentration of DSS.
A: Only when drinking DSS reach a certain threshold can it successfully induce enteritis. If the threshold is not reached, weight loss can occur, but the pathology shows no inflammation.
A: DSS is tolerated by animals over a period of time, so symptoms decrease to normal during the second cycle.
A: Spermine can remove the inhibition of PCR by DSS. References: Journal of Microbiological Methods, 2018 Jan, 144:1-7.
A: Give 2% of DSS solution to the stomach at 10:00 a.m., 14:00 p.m. and 18:00 p.m. respectively every day, 30ml/kg each time, no additional drinking water. Full amount of mixed formula granule feed was given after the last gavage every day, and the feed was taken away at 8:00 a.m. the next day. The modeling cycle time is 9 days. References: A comparative study of dextran sodium sulfate free drinking and quantitative gavage induced acute colitis model in mice, gastroenterology 2009,14 (1), 27-30.
A: There is a difference, C57 background is pure, induced enteritis is more stable. When using Babl/ C mice, preliminary experiments are suggested.
A: TNBS: Long course of inflammation. Large individual differences. Low similarity with ulcerative colitis.
A: : It is recommended to replace with new dss solation every 1-2 day. The cycle time of acute modeling is 5~7 days.
A: DSS concentration of 2~5% is the result that we summarized according to the previous literatures. We found that the concentrations of researchers within in this range.
A: Yes, reference: YAP triggers the Wnt/ -catenin signaling pathway and prime enterocyte self-renewal, regeneration and tumorigenesis after DSS-induced injury. Cell death and Disease, 2018(9):153.
A: The threshold of 30mg/g is the total intake rather than the daily intake. The formula is, Total drinking volume (ml) X [DSS (g)/100 ml])/Initial body weight (g).
A: It may be due to intestinal residual DSS, which inhibit the downstream experiment. Spermine is suggested for DSS removal. In addition, it may also be caused by inhibitors contained in feces, so the template concentration is suggested to be diluted before PCR. If dilution works, it is suggested to optimize the extraction procedure of fecal DNA and remove too inhibitors first.
A: Other functions of DSS: anticoagulation, lowering blood lipid, anti-virus, inhibiting effect on some enzymes, improving nucleic acid hybridization and cosmetic additives.
A: It is recommended to take 1~2cm near the anal segment.
A: References: Gastroenterology, 2002, 123(1):256-270; J Immunol, 2004, 172(9): 5664-5675; Journal of Gastroenterology and Hepatology 2013, 22(12): 1221-1224; Inflammatory.
A: The expression of tumor necrosis factor, interleukin-interferon, IL-10 and IL-12 increased on the first day of DSS solution administration and gradually increased with the time of administration. The expression of IL-and IL-mrna was increased in acute DSS colitis model. Th2-cell-mediated cytokines IL-4 and IL-10 also play an important role in the chronic DSS colitis model.
A: Literature suggests that mice weight should be not less than 16~18g, and the mice should be selected according to week age. 8~12 weeks is recommended, and the weight is about 22g.
A: Body weight and DAI index are not related to the intake threshold, which means the blood in the feces is not synchronized with the pathology.
A: Please check whether there is no significant difference in the amount of water the animals drink each day, and check all animals have repeated weight or some animals only. It has to do with individual differences in animals.